In vitro antiplasmodial activity and cytotoxicity of. Moreover, all plant extracts were found to have in vitro antiplasmodial activity. Antiplasmodial activity and toxicity of crude extracts. The in vitro antiplasmodial activity of seven etoh extracts and twenty fractions from the partition of the initial ethanolic extracts from seven african medicinal plants used in the democratic.
Preliminary evaluation of selected medicinal plants for. Antiplasmodial properties of some malaysian medicinal plants. Antiplasmodial activities of crude extract and solvent fractions were. John oludele olanlokun, oluwole moses david, anthony jide afolayan. From the studies conducted on the plant until now, it is known that the methanolic root extract of the plant has schizonticidal activity as high as 84. The data revealed that significant reduction in growth of fungal strain f. Thus, the present study aims at evaluating the invivo antiplasmodial activity of solvent fractions of methanolic root extract of. The two most active extracts that produced 100% inhibition of the parasite growth at concentration of plant material. Antimalarials from plant pathogenic fungi by mallika. Antiplasmodial activity of vernonia adoensis aqueous. In vitro assessment of the antiplasmodial activity of three plants. Both plant extracts also demonstrated antiplasmodial activity against the chloroquine resistant plasmodial parasites. Facilitate digestion the leaves of the papaya plants contain. Antiplasmodial, antitrypanosomal, antileishmanial and.
The results from the in vitro antiplasmodial testing of 7 etoh extracts and 20 fractions from the partition of their respective initial etoh extracts are presented in table 2. The in vitro antiplasmodial activity and cytotoxicity of extracts from jatropha tanjorensis leaves was evaluated. Evaluation of the antiplasmodial activity of extracts of. Bacterial diversity and community structure in typical plant rhizosphere. The present study investigates the antimalarial activity of the methanolic extracts. The antiplasmodial activities were carried out using the pldh assay to plasmodium falciparum d10 strain sensitive strain while the cytotoxic activities were carried out towards madindarby bovine kidney mdbk cells using mtt assay. The extracts of experimental plants were evaluated for their antiplasmodial activity against 3d7 and indo strains of p. Continuous in vitro cultures of asexual erythrocytes stages of p. Antimalarial and antiplasmodial activity papaya leaves are made into tea as a treatment for malaria.
The current study was undertaken to evaluate the antiplasmodial activity and toxicity of the organic extracts of cassia alata. Annickia enantia kummeriae annonaceae, artemisia annua asteraceae, pseudospondias microcarpa anacardiaceae, drypetes natalensis euphorbiaceae, acridocarpus chloropterus malpighiaceae, maytenus senegalensis celastraceae and. The use of plant extracts with known antimicrobial properties, can be of great significance in therapeutic treatments. The plant leaves were successively extracted into three 3 extract forms including aqueous extract, ethanolic extract and hydroethanolic 50. Antimicrobial activity of selected medicinal plants. The proliferation and resistance of microorganisms area serious threat against humankind and the search for new therapeutics is needed. Of these, extracts from artabotrys crassifolius, pericampylus glacus and leuconotis eugeniifolia showed promising antiplasmodial activities at ic 50 of 15. In vitro antiplasmodial activity and phytochemicals.
In vitro antiplasmodial activity of central american. For quality control, high performance thin layer chromatography hptlc was performed on extracts. The plant extract was prepared by maceration in methanol. The antiplasmodial activity of extracts of these plants was evaluated in vitro using the multiresistant strain w2 of plasmodium falciparum. Plant extract showed concentration dependent antioxidant activity and ferric reducing power. Drugs and plant extracts were then placed in 96well flat bottom tissue culture grade plates. Antiplasmodial activity of solvent fractions of methanolic. Herein, the in vitro and in vivo antiplasmodial activity and cytotoxicity of medicinal plant extracts were investigated. European scientific journal april 2016 edition vol. Seven of the eleven extracts exhibited significant antiplasmodial activity ic 50 edulis, c. In previous study on antimalarial plants from mali 31 crude extracts of a. Data analysis using twoway anova to determine the significant interactions between plant extracts and.
Evaluation of antiplasmodial activity of medicinal plants. In the in vivo evaluation of the antiplasmodial activity of the plant extracts was employed against. Antiplasmodial activity of methanol leaf extract of citrus. Selected plants being used in indian traditional medicine to treat malaria and malarialike symptoms plant name, family, part used common name method of preparation referencs adhatoda zeylanica medic. Larvicidal, antipyretic and antiplasmodial activity of. Preliminary evaluation of selected medicinal plants for antiplasmodial activity 40 table 3. Various extracts obtained from 19 plants collected in kenya were tested for antiplasmodial activity. Antibacterial activity of alkaloid extracts from ochrosia. Antibacterial activity of plant extracts 247 brazilian journal of microbiology 2000 31. With regard to the ethanolic extract a, a high antiplasmodial activity ic 50 pdf antiplasmodial activity of plant extracts used in west african traditional medicine five plants originating from ivory coast were selected after an ethnobotanical survey, alchornea. Antiplasmodial activity and phytochemical analysis of extracts from selected ugandan medicinal plants.
In nigeria, malaria is a major public health problem and there are high cost of the effective antimalarial drugs, poor quality drugs and increased emergence of plasmodial resistance. Thus, it is worth documenting the ethnobotanical information and testing the antiplasmodial activity of plant extracts. The results showed antioxidant activity using dpph were found to be increased in a concentration dependent. M as antiplasmodial activity when tested in vitro against plasmodium falciparum. Antiplasmodial activity of vernonia adoensis aqueous, methanol. This suggests that leaves should be preferred over the whole plant and extraction should be made in ethyl acetate for enriching antiplasmodial molecules. All plant samples displayed various antiplasmodial activity. The methanolic extract phyllanthus niruri stone breaker showed the maximum activity against staphylococcus sp. Antiplasmodial activity of the ethnobotanical plant.
Pdf antiplasmodial activity and phytochemical analysis of extracts. In this study the antiplasmodial effect of the leaf crude extract of v. The plant leaves were successively extracted into three 3 extract forms aqueous extract, ethanolic extract and hydroethanolic 50. Pdf in vitro antimalarial activity of medicinal plant. Lipophilic extracts of piper hispidum, siparuna andina, s.
The antimalarial potential of aqueous leaf extracts of cymbopogon citratus was investigated in this present study. In sudan, out of 21 compounds isolated from 9 medicinal plants used in traditional medicine, only gedunin and quercetin showed ic50 of 1. Antioxidant activity of the plant extracts were characterized by using dpph free radical scavenging method. Antiplasmodial activity of medicinal plant preparations. Promising antiplasmodial activity was found in the extracts from two plants, phyllanthus emblica leaf 50% inhibitory concentration ic 3d7. The cytotoxic activity of the extracts of cocculus hirsutus on mcf7 cells was investigated in vitro through mtt assay.
In vitro antimalarial activity of medicinal plant extracts against plasmodium falciparum. The results of antimicrobial activity showed that water and ethanol crude extracts were active on all the strains of pathogens tested at different concentrations, with ethanol extracts exerting more activity. Plant extracts showed moderate to good antiparasitic effects. This study revealed for the first time the antiplasmodial activity of the plant c. Plant extracts were correspondingly subjected to an antiproliferative bioassay against mammalian skeletal myoblast cells. A preliminary screening of potentially antimalarial plants.
The antiplasmodial, antitrypanosomal and antileishmanial activity of 25 plant extracts obtained from seven tanzanian medicinal plants. The methanolic leaf extract of ricinus communis is a low toxic plant with antiplasmodial activity which can be exploited as a potential source of new antimalarial drug to combat malaria. Various phytotoxins released by necrotropic plant pathogenic fungi may inhibit the plantlike metabolic pathways in the apicoplast, a chloroplastlike organelle, essential for the survival of apicomplexan parasites, such as plasmodium which cause malaria in humans. Antiplasmodial potential of medicinal plant extracts from. Evaluation of antimalarial activity of hydromethanolic crude extract a. In vitro antiplasmodial activity and prophylactic potentials of extract. Cytotoxycity and antiplasmodial activity of phenolic. But all the mice treated with cq survived for 3 months additional file 1. The in vitro antiplasmodial activity of seven etoh extracts and twenty fractions from the partition of the initial ethanolic extracts from seven african medicinal plants used in the democratic republic of congo dr congo for the treatment of malaria was evaluated. Plant extract exhibited no signs of toxicity on mice at a dose of 5000.
The structures of isolated compounds were elucidated using chemical and spectroscopic methods. Further studies are warranted to explore its efficacies and lead compounds of these three plant extracts for the. Promising antiplasmodial activity was found in the extracts from two plants, phyllanthus emblica leaf. In vitro and in vivo antimalarial activity of plants from. The emergence and spread of plasmodium falciparum with resistance to chloroquine cq, the safest and cheapest antimalarial drug, coupled with the increasing cost of alternative drugs especially in developing countries have necessitated the urgent need to tap the potential of plants for novel antimalarials. In vitro antiplasmodial activity of dysoxylum caulostachyum miq.
In vitro antiplasmodial activity the plant extracts were tested in triplicate against chloroquine sensitive cqs strain of p. The plants are usually used to treat the malaria disease, and the people commonly use the extract of this plants for this purpose 5. In vitro and in vivo antiplasmodial activities of gynura. Three plant extracts caused 100% inhibition of the parasite growth at concentrations of plant material. In vivo and in vitro antimalarial activity of vernonia. Antiplasmodial activity of selected medicinal plants. Antiplasmodial activity was assessed 1 corresponding author. Antiplasmodial activity of methanol leaf extract of citrus aurantifolia christm swingle. The 50% inhibitory concentration ic50 values of the crude aqueous extracts were found to be 2. Nmrbased metabolomic study of medicinal plants used. In vitro antiplasmodial activities and synergistic. The in vitro cytotoxicity assay of the 6 plant crude extracts using kb cells indicated that t. The selected plants, acokanthera oppositifolia lam apocynaceae is a species encountered in eastern.
Thus, there is a need for alternative source of medicine in malaria treatment and prevention. Pdf antiplasmodial activity and phytochemical analysis. The plant extracts analysed in this study presented an average antiplasmodial activity 58%. The results of the 12 fractions of three of the bioactive plant extracts in the in vitro antiplasmodial assay. Other related study reported that screening of the appropriate extracts of ochrosia maculata elicited. Antiplasmodial activity of aqueous leaf extract of. In vitro antiplasmodial assays on plasmodium falciparum in human red blood cell culture. The purpose of this study was to discover antimalarial compounds from necrotropic plant pathogenic fungi. Among the various extracts, the lowest 50% inhibitory concentrations ic50 of 24. Antifungal activity of six botanical extracts was assayed and the effect of plant extracts on the growth of fungal strain f. This is the first scientific report to document significant antiplasmodial activity of extracts from t.
Antiplasmodial activity of extracts of tridax procumbens and phyllanthus amarus in in vitro plasmodium falciparum culture systems. In vitro antiplasmodial and cytotoxic activities of. Results in vitro antiplasmodial activity and cytotoxicity of aqueous extracts all five aqueous extracts showed activity against the c h l o r o q u i n e s e n s i t i v e 3 d 7 p. Quantitative assessment of antiplasmodial activity in. Antiplasmodial compounds from leaves of dodonaea angustifolia. Plant extract achieved a maximum antioxidant activity of 4% within 40 min. Data analysis using twoway anova to determine the significant interactions between plant extracts and plasmodic growth. In vitro antiplasmodial activity of extracts and fractions. Antibacterial activity of plants 719 effective extracts are considered, e.
Evaluation of antiplasmodial activity of extracts and constituents. All extracts and pure compounds were tested for antiplasmodial activity in which the hexane bark extract showed highest activity with ic 50 0. In vitro antiplasmodial activity of aqueous and organic extracts from moringa ovalifolia was measured using parasitaemia posttreatment with plant extracts as well as the ic50 values. The tannins extracts showed moderate antiplasmodial activity on both p. In vitro antiplasmodial activity and toxicity assessment of plant. Nmrbased metabolomic study of medicinal plants used against malaria and the isolation of bioactive alkaloids.
Okundoperoxide, a bicyclic cyclofarnesylsesquiterpene. Summary the in vitro antiplasmodial activities of 14 plant species traditionally used in central america for the treatment of malaria or fever were evaluated. The antiplasmodial activity of the extracts and pure compounds was evaluated using the standard peters fourday suppressive method. Sixtynine extracts from 11 plant species were prepared and screened for in vitro activity against p. Six methanol meoh extracts and one chloroform extract showed in vitro antiplasmodial activity against the d6 plasmodium falciparum strain, while only three.
Evaluation of the antiplasmodial activity and lethality of. Evaluation of the antiplasmodial properties of namibian. Antioxidant and in vitro cytotoxic activity of extracts of. The present report describes the antiplasmodial and anticancer activities of samples isolated from the methanol extract of albizia zygia mimosaseae. The plant extracts were obtained from forest research institute malaysia frim.
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